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《Journal of Jimei University(Natural Science)》 2010-03
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Antibacterial Activities in Different Tissues or Organs of Japanese Eel,Anguilla japonica

XU Tong-ling1,GUAN Rui-zhang1,2,LIANG Ying1,2,HUANG Wen-shu1,2(1.Fisheries College,Jimei University,Xiamen 361021,China;2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University) ,Fujian Province,Xiamen 361021,China)  
Compared and analysed of the antibacterial activities in different tissues and organs,including liver,gill,spleen,bile,kidney and serum of A.japonica.This study obtained acid extractions of the tissues and organs from Japanese eels.Using agar diffusion method,the antibacterial activity of those extractions against four common pathogenic bacteria(Enterobacter spp.B01,Edwardsiella tarda B09,Aeromonas spp.B18 and A.hydrophila B27) from the cultivated eels was detected.The results showed that antibacterial activities of liver,gill,spleen,bile were obvious.The extractions of kidney exhibited antibacterial activity against B18,B27 and B01.The extractions of serum exhibited antibacterial activity against B18.The antibacterial activities of extractions from different tissues and organs were different to the same bacterium.The antibacterial activity of liver was the highest to all experimented bacteria.The antibacterial activity of extractions from the same tissues or organs varied to different bacteria.The acid extractions were further purified by the method of reverse-phase liquid chromatography on Source 5R RPC-ST-4.6/150 column(Amersham Bioscience,Sweden) .The results showed that the killing indexes of the extractions from liver,kidney,gill and bile were higher than 50 % when eluted by the concentration of aceonitrile ranging from 19.4 % to 35.4 %,while the killing index of extractions from serum and kidney were lower than 50 % when eluted by the concentration of aceonitrile ranging from 0 % to 80 %.
【Fund】: 農業部公益性行業(農業)科研專項(nyhyzx07-043);; 福建省科技廳高校專項(2007F5079);; 集美大學博士啟動基金項目(ZQ2006024)
【CateGory Index】: S917.4
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