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《Journal of Jimei University(Natural Science)》 2010-02
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Study on Free-radical Scavenging Activities of Polysaccharide Extracts from Longan

WANG Xue-yan,CHEN Fa-he,WU Guang-bin,HUANG Zhi-yong(School of Biotechnology Engineering,Jimei University,Xiamen 361021,China)  
The effect of purification technique on free-radical scavenging activities of polysaccharide extracts from longan was compared towards 1,1-diphenyl-2-picrylhydrazyl radical(DPPH·),hydroxyl radical(·OH) and superoxide anion radical(O-_2·).With ascorbic acid as comparison,the scavenging abilities were determined by spectrophotometry against DPPH·,·OH produced by Fenton reaction and O-_2· generated by pyrogallol autoxidation of the crude longan polysaccharide(CLPS),semi-pure longan polysaccharide product(SLPS) prepared by removing its protein with the Sevag method,semi-pure longan polysaccharide product(TLPS) prepared by removing its protein with the TCA method,pure longan polysaccharide(LPS-2)purified by DEAE-Cellulose 52 ion-exchange chromatography,respectively.The results showed that the higher the purity of polysaccharide,the stronger the scavenging activity to DPPH·,and the order of activity from greatness to weakness was:LPS-2 TLPS SLPS CLPS;in addition,as for ·OH and O-_2,the activity of SLPS was the strongest and the LPS-2 was the weakest,which implied that the motley protein inhibited the scavenging activities to ·OH and O-_2·.Nevertheless the protein moiety in glycoprotein promoted the activities.
【Fund】: 福建省自然科學基金資助項目(B0510026);; 福建省科技計劃重點項目(2007N0046)
【CateGory Index】: R285
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