Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of New Medicine》 2019-02
Add to Favorite Get Latest Update

Expression of let-7c-5p and miR-122-5p in Nonstructural Protein I of Respiratory Syncytial Virus Infected A549 Cells

Zeng Dongxin;Feng Shuwen;Wang Shouyi;Department of Pediatrics,Zhongnan Hospital of Wuhan University;  
Objective To study the alteration of let-7c-5p and miR-122-5p expression in A549 cells after infected with nonstructural protein I(NS1)of respiratory syncytial virus(RSV).Methods We cultured A549 cells to establish the infection model of NS1, and the control of empty vector group was also set up.Cells after 24 h and 72 h infection were collected and real-time PCR was performed to evaluate the alteration of let-7c-5p and miR-122-5p expression.Target gene prediction and function analysis were performed using bioinformatics software. Results Let-7c-5p expression elevated while miR-122-5p expression decreased in NS1 infection group.Bioinformatics software predicted that target genes focused on apoptosis and immune response.Conclusion NS1 probably regulate cell apoptosis and suppress immune response by upregulating the expression of let-7c-5p and downregulating the expression of miR-122-5p.
【Fund】: 國家自然科學基金資助(81670007)
【CateGory Index】: R373
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 ZHAO Meng;JIANG Yong;YANG Pu;ZHENG Jun-wen;XIE Meng;ZHAO Dong-chi;Department of Pediatrics, Zhongnan Hospital of Wuhan University;;Study on the mechanisms of apoptotic regulation by respiratory syncytial virus non-structural proteins[J];生物醫學工程與臨床;2015-01
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 Zeng Dongxin;Feng Shuwen;Wang Shouyi;Department of Pediatrics,Zhongnan Hospital of Wuhan University;;Expression of let-7c-5p and miR-122-5p in Nonstructural Protein I of Respiratory Syncytial Virus Infected A549 Cells[J];醫學新知雜志;2019-02
2 Wang Honggang;Xu Xinxin;Chen Chengbin;Wang Chunguo;Chen Li;Song Wenqin;Bai Yanling;Zhang Jinhong;Liu Fang;Biological Experimental Teaching Center,Nankai University;;Exploration on testing methods of apoptosis in basic experimental teaching[J];實驗技術與管理;2015-11
【Secondary Citations】
Chinese Journal Full-text Database 2 Hits
1 Bu Xuanyun(College of Life Sciences,Kunming University of Science and Technology,Kunming 650224);Research Development of RSV Nonstructural Proteins NS1 and NS2[J];生物技術通報;2011-05
2 PENG Hui-qin, YE Zhao-ming, QIAN Jing, TAO Jun (Zhejiang University School of Medicine, Hangzhou 310031, China);Effect of respiratory syncytial virus on apoptosis and expressions of FasL, Fas, Bcl-2 and Bax[J];中國病理生理雜志;2005-09
?2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved
在线中文字幕亚洲日韩_欧美 亚洲 中文字幕 高清_亚洲 日韩 中文 综合AV <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>