Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xuzhou Medical University》 2019-03
Add to Favorite Get Latest Update

Study on tissue engineering skin for chemical irritation detection

ZHANG Guangjing;WANG Zhengxiang;FENG Shijun;AI Dongfang;Department of Dermatology,Cangzhou Central Hospital;  
Objective To undergo chemical irritation detection using a tissue engineering skin model established in vitro. Methods The tissue engineering skin model was constructed using bovine type Ⅰ collagen as scaffold and human normal skin keratinocytes and fibroblasts as cell sources. Twenty chemicals were selected as test substances from the substances specified by the European centre for validation of alternative experiments for skin irritation. During the detection,25 μl of 20 chemicals(25 mg solid were ground into powder before added into 25 μl distilled water) were contacted with the surface of the test model for 15 min. The cell viability was measured by MTT. Meanwhile, 25 μl distilled water was added to the negative control and 25 μl of 5 % sodium laurate was added to the positive control. Results Based on the relative cell viability values, 16 chemicals were identified by the reconstructed detection model. Conclusions The tissue engineering skin model determines the irritation of chemicals and can be used as a detection method for chemical irritation.
【CateGory Index】: TQ652
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Jin Yan, Lu Hong bing, Liu Yuan, et al.College of Stomatology, Fourth Military Medical University, Xi'an 710032 〔;Development of human tissue-engineered skin with full thickness[J];實用口腔醫學雜志;2002-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Guangjing;WANG Zhengxiang;FENG Shijun;AI Dongfang;Department of Dermatology,Cangzhou Central Hospital;;Study on tissue engineering skin for chemical irritation detection[J];徐州醫科大學學報;2019-03
2 Zhang Guangfu;Liu Wei;Xing Liguo;XuWeichang;Jin Yihe;Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education, School of Environmental Science and Technology, Dalian University of Technology;Shenyang Research Institute of Chemical Industry of SINOCHEM GROUP;;Using Human Skin Model to Evaluate Skin Corrosion/Irritation of Fungicide[J];生態毒理學報;2014-03
3 QIU Xue-wen,WANG Jia-han,REN Jia-liang,LI Zhi-qing(Department of Burns,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China);Clinical observation on the repairing of residual burn wounds with tissue engineering skin[J];中國美容醫學;2013-01
4 LIU Xiao-li,WANG Li-Feng,WANG Yang Tianjin University of TCM,Tianjin China 300193;Research Progress on Construction of Tissue Engineered Skin and Application of Percutaneous Absorption[J];甘肅中醫;2010-05
5 Liu Hai-ping1,Wang Hong1,Dong Xiao-hua2,Yang Hua1 1Department of Burns,Second Affiliated Hospital,Kunming Medical College,Burn Research Center of Yunnan Province,Kunming 650000,Yunnan Province,China;2Department of Cardiology,First Affiliated Hospital,Kunming Medical College,Kunming 650000,Yunnan Province,China;Significance of endothelial progenitor cells for tissue engineered skin construction[J];中國組織工程研究與臨床康復;2010-15
6 LI Yuan1,LU Wei1,2,JI Kun2,LIU Yuan1,DONG Shao-Zhong1,JIN Yan1,2 1Department of Oral Histopathology,School of Stomatology,2Research and Development Center for Tissue Engineering,School of Stomatology,Fourth Military Medical University,Xi'an 710033,China;Experimental research of histological difference between tissue-engineered skin and normal human skin[J];第四軍醫大學學報;2009-24
7 Chen Jian-ping, Liu De-wu, Mao Yuan-guiDepartment of Burns, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China;Structure and properties of tissue-engineered skin[J];中國組織工程研究與臨床康復;2008-24
8 XIAO Houan,JIN Yan,WU Zhouhu,NIE Xin,ZHANG Yongjie,LU Meiru,LIU Yaling.Department of Burns and Plastic Surgery,Xi'an Railway Central Hospital,Xi'an 710054,China;The clinic application of tissue engineered active skin on the diabetic ulcer[J];中華損傷與修復雜志(電子版);2008-02
9 QIN Rui-feng NIE Xin ZHANG Yong-jie JIN Yan (1 College of Stomatology,Fourth Military Medical University,Xi'an 710032,China;2 Daping Hospital,Third Military Medical University,Chongqin 400042,China);Clinical Effect Assessment of Tissue-engineered Composite Skin on the Donor Site Defect Wound[J];現代生物醫學進展;2007-12
10 ZHANG Lin-pu.(The Central Laboratory of the Tianjin Stomatological Hospital,Tianjin 300041,China);Study Advancement of Transplantation Immunological Problems of Seeding Cells in Tissue Engineering[J];醫學綜述;2007-21
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 YANG Jun,XIAO Shi chu,XIA Zhao fan (Department of burn,Changhai hospital,Shanghai 200433,China);Progress in the field of burn dressing[J];國外醫學.生物醫學工程分冊;2001-05
2 WENG Yu lai 1,3,4 , CAO Yi lin 2,3,4 ,CAI Xia 2,3 , Vacanti CA 4 .(1. Department of Oral Medicine, Shanghai Ninth People' s Hospital,Shanghai Second Medical University,Shanghai 200011,China;2.Department of Plastic Reconstructive Surgery,;Autologous Tissue Engineered Skin for Full Thickness Skin Replacement[J];上海口腔醫學;2000-03
3 WANG Zi min, JIAO Xiang yang, XING Xin.Department of Plastic Surgery, Changhai Hospital, the Second Military Medical University. Shanghai, P.R.China 200433.E mail:rochies 424@sh.163.net;TISSUE ENGINEERING OF SKIN[J];中國修復重建外科雜志;2000-04
4 Wu Jinjin; Liu Rongqing; Ye Qingyi; Tang Shuqian; Zhong Baiyu (Department of Dermatology,Southwest Hospital, Third Military Medical University, Chongqing 4000038);A Model of Artificial Skin Estabished by Three-dimension Culfure[J];重慶醫學;1999-04
?2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved
在线中文字幕亚洲日韩_欧美 亚洲 中文字幕 高清_亚洲 日韩 中文 综合AV <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>